Data/hora: 08/06/2024 11:35:08

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Production and characterization of amylases from Zea mays malt	
Autores: 	Biazus,Joana Paula Menezes; Souza,Roberto Rodrigues de; Márquez,Jesus Espinoza; Franco,Telma Teixeira; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.	
Data: 	2009-08-01	
Ano: 	2009	
Palavras-chave: 	Maize seed; Malting; Α and β-amylase; Biochemistry characterization; Germination model.	
Resumo: 	In this work the α and β-amylase enzymes were obtained from maize (Zea mays) malt and were biochemistry characterized. A germination study to obtain the maize malt with good amylase activity was made. The maize seeds were germinated in laboratory and the enzymatic activity was measured daily. Activity dependence to germination time were fitted to an exponential model (A=A0eµt), which showed that the behaviour of enzymatic activity in the germination process was similar to the growth of the microorganism. Its model could be applied to describe the mechanism of α-amylase production for each maize varieties and others cereals. Maize malt characterization showed that α and β-amylase had optimal pH between 4-6.5, optimal temperature 50 and 90ºC, and molecular weight of 67.4 and 47.5kDa, respectively. This work contributed with the advances in biotechnology generating of conditions for application of a new and of low price amylases source.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400024	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132009000400024	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.52 n.4 2009	
Fonte: 	info:eu-repo/semantics/openAccess