Data/hora: 11/06/2024 06:43:47

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Performance evaluation of CHO-K1 cell in culture medium supplemented with hemolymph	
Autores: 	Raffoul,Tássia; Swiech,Kamilla; Arantes,Mabel Karina; Sousa,Álvaro Paiva Braga de; Mendonça,Ronaldo Zucatelli; Pereira,Carlos Augusto; Suazo,Cláudio Alberto Torres.	
Data: 	2005-06-01	
Ano: 	2005	
Palavras-chave: 	Animal cell; CHO-K1; Microcarrier; Growth; Attachment; Hemolymph.	
Resumo: 	The aim of this work was to evaluate the potential of hemolymph utilization as a culture medium supplement to cultivate the animal cell CHO-K1. For this purpose 1% v/v of hemolymph was added to DMEM medium containing 10% v/v of FBS and 1 or 4.5 g/L of glucose. The culture was grown in spinner flasks incubated in a 10% v/v CO2 environment, at 37ºC, with the Cytodex 1 microcarrier. Comparing the results obtained from the culture with hemolymph against those without hemolymph, a positive influence of the hemolymph was observed, as the experiment with hemolymph presented a 52% higher cell concentration and a higher productivity of up to 40%.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400011	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132005000400011	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.48 n.spe 2005	
Fonte: 	info:eu-repo/semantics/openAccess