Data/hora: 11/06/2024 12:12:00

Provedor de dados: | 52
País: | Brazil
Título: | Performance evaluation of CHO-K1 cell in culture medium supplemented with hemolymph
Autores: | Raffoul,Tássia; Swiech,Kamilla; Arantes,Mabel Karina; Sousa,Álvaro Paiva Braga de; Mendonça,Ronaldo Zucatelli; Pereira,Carlos Augusto; Suazo,Cláudio Alberto Torres.
Data: | 2005-06-01
Ano: | 2005
Palavras-chave: | Animal cell; CHO-K1; Microcarrier; Growth; Attachment; Hemolymph.
Resumo: | The aim of this work was to evaluate the potential of hemolymph utilization as a culture medium supplement to cultivate the animal cell CHO-K1. For this purpose 1% v/v of hemolymph was added to DMEM medium containing 10% v/v of FBS and 1 or 4.5 g/L of glucose. The culture was grown in spinner flasks incubated in a 10% v/v CO2 environment, at 37ºC, with the Cytodex 1 microcarrier. Comparing the results obtained from the culture with hemolymph against those without hemolymph, a positive influence of the hemolymph was observed, as the experiment with hemolymph presented a 52% higher cell concentration and a higher productivity of up to 40%.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400011
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132005000400011
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.48 n.spe 2005
Fonte: | info:eu-repo/semantics/openAccess