Data/hora: 01/06/2024 08:05:08

Provedor de dados: | 52
País: | Brazil
Título: | Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysis
Autores: | Oliveira,Antonio Martins; Oliva Neto,Pedro de.
Data: | 2011-10-01
Ano: | 2011
Palavras-chave: | Autolysis; NaCl plasmolysis; Yeast extract; RNA extraction; Nucleotides.
Resumo: | The optimization of autolysis of Saccharomyces cerevisiae from brewery was studied aiming at the maximum ribonucleic acid extraction and yeast extract production. The best conditions for yeast autolysis was 55.2ºC, pH= 5.1 and 9.8% NaCl for 24h of processing, without the NH3 use. In these conditions, the RNA yield was 89.7%, resulting in 51.3% of dehydrated yeast extract with 57.9% protein. The use of 12.2% NH3 at 60ºC after autolysis (8h) and plasmolysis (8h) was not viable due to the reduction in the RNA yield from 89.7to78.4%. On the other hand, the thermal shock at 60ºC for 15 minutes prior to autolysis provided an increase in the yield from 89.7 to 91.4%. The autolysis, including NaCl plasmolysis in the optimized conditions was efficient, economic and with short time, thus usable for industrial purpose to obtain more valuable products such as yeast extract enriched in RNA and/or protein, for different applications.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500019
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132011000500019
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.54 n.5 2011
Fonte: | info:eu-repo/semantics/openAccess