Data/hora: 01/06/2024 04:41:16

Provedor de dados: | 52
País: | Brazil
Título: | Biotechnological potential of alternative carbon sources for production of pectinases by Rhizopus microsporus var. rhizopodiformis
Autores: | Damásio,André Ricardo de Lima; Maller,Alexandre; Silva,Tony Márcio da; Jorge,João Atílio; Terenzi,Hector Francisco; Polizeli,Maria de Lourdes Teixeira de Moraes.
Data: | 2011-02-01
Ano: | 2011
Palavras-chave: | Agro industrial residues; Lemon peel; Pectin lyase; Polygalacturonase; R. microsporus var. rhizopodiformis.
Resumo: | Fungi collected from Brazilian soil and decomposing plants were screened for pectinase production. R. microsporus var. rhizopodiformis was the best producer and was selected to evaluate the pectic enzyme production under several nutritional and environmental conditions. The pectinase production was studied at 40ºC, under 28 carbon sources-supplemented medium. The inducer effect of several agro-industrial residues such as sugar cane bagasse, wheat flour and corncob on polygalacturonase (PG) activity was 4-, 3- and 2-fold higher than the control (pectin). In glucose-medium, a constitutive pectin lyase (PL) activity was detected. The results demonstrated that R. microsporus produced high levels of PG (57.7 U/mg) and PL (88.6 U/mg) in lemon peel-medium. PG had optimum temperature at 65 ºC and was totally stable at 55 ºC for 90 min. Half-life at 70 ºC was 68 min. These results suggested that the versatility of waste carbon sources utilization by R. microsporus, produce pectic enzymes, which could be useful to reduce production costs and environmental impacts related to the waste disposal.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100019
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132011000100019
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.54 n.1 2011
Fonte: | info:eu-repo/semantics/openAccess