Data/hora: 09/06/2024 19:06:39

Provedor de dados: | 52
País: | Brazil
Título: | Chemotaxonomic Fingerprinting of Chilean Lichens Through Maldi and Electrospray Ionization Mass Spectrometry
Autores: | Santos,Leonardo Silva; Soriano,Maria del Pilar Camarantin; Mirabal-Gallardo,Yaneris; Carrasco-Sanchez,Veronica; Nachtigall,Fabiane Manke; Pereira,Iris; Pereira,Eugenia.
Data: | 2015-04-01
Ano: | 2015
Palavras-chave: | Chilean lichens; Chemotaxonomic; Fingerprint; MALDI-TOF-MS; UPLC-ESI(-)-MS.
Resumo: | The aim of this work was to study a fast, new, sensitive, and simple method for the chemotaxonomic classification of Chilean lichens (Teloschistes chrysophthalmus, Ramalina farinacea, Usnea pusilla, Ramalina chilensis and Stereocaulon ramulosum) using MALDI-TOF-MS and UPLC-ESI(-)-MS data. Lichens soluble proteins fingerprints were acquired by MALDI-TOF-MS and they were analyzed by chemometric (PCA). Lichens organic extracts fingerprints were obtained by UPLC-ESI(-)-MS. MALDI-TOF-MS associated with chemometric analysis was used to detect new m/z patterns of soluble proteins that were compared with Protein Data Bank of UnitPro. These data also permitted the satisfactory distinction among the families and species. UPLC-ESI(-)-MS fingerprints analyses of the organic extracts showed the presence of five major lichen compounds (atranorin, parietin, teloschistin, ramalinolic and usnic acids). In contrast to other techniques, MALDI-TOF-MS associated with chemometric analysis and UPLC-ESI(-)-MS provided a new, fast and sensitive method for chemotaxonomic characterization of lichens.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200244
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-8913201400185
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.58 n.2 2015
Fonte: | info:eu-repo/semantics/openAccess