Data/hora: 06/06/2024 00:19:56

Provedor de dados: | 52
País: | Brazil
Título: | Micropropagation of an elite F1 watermelon (Citrullus lanatus) hybrid from the shoot tip of field grown plants
Autores: | Khalekuzzaman,Mohammad; Khatun,Marufa; Rashid,Mohammad Harunur; Sheikh,Mominul Islam; Sharmin,Shamima Akhtar; Alam,Iftekhar.
Data: | 2012-06-01
Ano: | 2012
Palavras-chave: | In vitro; Watermelon; Micropropagation; Hybrid vigor.
Resumo: | The aim of this work was to develop a protocol for rapid micropropagation of an elite F1 hybrid watermelon cultivar using shoot tip of field-grown plants. Maximum frequency (73%) of shoot tip showed growth response in MS medium supplemented with 5 mg l-1 benzyl adenine (BA) and 0.1 mg l-1 indole-3 acetic acid (IAA). Upon transfer to cytokinin-enriched medium, the cultures produced multiple shoots and 2.0 mg l-1 BA was optimum in this respect. Addition of gibberellic acid (GA3) in the multiplication medium resulted in better growth of shoots. Rooting rate was 100% when shoots were obtained from second subculture were cultured in medium with 1.0 mg l-1 indole-3 butyric acid (IBA). The shoots produced more roots with increasing number of subcultures. About 72% of the regenerated plantlets acclimatized successfully and survived in the soil condition.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300002
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132012000300002
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.55 n.3 2012
Fonte: | info:eu-repo/semantics/openAccess