Data/hora: 15/06/2024 14:32:12

Provedor de dados: | 52
País: | Brazil
Título: | Effect of carbon source on morphology and histodifferentiation of Araucaria angustifolia embryogenic cultures
Autores: | Steiner,Neusa; Vieira,Felipe do Nascimento; Maldonado,Sara; Guerra,Miguel Pedro.
Data: | 2005-11-01
Ano: | 2005
Palavras-chave: | Cell competence; Somatic embryogenesis; Carbohydrate; Conifers.
Resumo: | The aim of the present work was to establish in vitro conditions for the induction, stabilization and proliferation of embryogenic cultures of A. angustifolia. Pre-cotyledonary staged zygotic embryos inoculated BM medium supplemented with 5 µM 2,4- D, 2 µM BAP and Kin, and 3% maltose or sucrose resulted in 66.7% induction rate. The rate of induction of embryogenic cultures was affected by the carbon source, as well the multiplication and morphology of the embryogenic cultures. Embryogenic cultures maintained in BM medium with maltose presented bipolar morphology. Globular somatic embryos were obtained BM medium with 9% (PEG) and (9%) maltose. These results could establish an in vitro regenerative protocol towards the conservation and improvement of this important species.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800005
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132005000800005
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.48 n.6 2005
Fonte: | info:eu-repo/semantics/openAccess