Data/hora: 06/06/2024 08:11:37

Provedor de dados: | 52
País: | Brazil
Título: | Production of pectinases by A. niger: influence of fermentation conditions
Autores: | Martos,María A.; Martinez Vazquez,Francisco; Benassi,Fernando O.; Hours,Roque A.
Data: | 2009-06-01
Ano: | 2009
Palavras-chave: | Pectinases; Polygalacturonase; Pectinesterase; Aspergillus niger; Response surface methodology; Citrus pectin.
Resumo: | Response surface methodology was used for optimization of polygalacturonase (PG) and pectinesterase (PE) production in submerged fermentation by A.niger. A Central Composite Experimental Design was applied, consisting of 22 experiments, including eight central points. Variables studied were: fermentation time (24 to 120 h), pH (3.5 to 6.5) and initial concentration of pectin (5 to 20 g/l). Maximum PE production was 220 U/l, after 74 h of culture, in a medium containing 20 g/l of pectin (pH 6.5). The optimal conditions for PG production were pH: 4.1, 20 g/l of pectin and 94 h of fermentation with a maximum value of 1032 U/l. Under these conditions, the PE production was low (15 U/l). A liquid extract with high PG activity and low PE activity could be suitable to be used in food processing in order to reduce the production of methanol.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300007
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132009000300007
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.52 n.3 2009
Fonte: | info:eu-repo/semantics/openAccess