Data/hora: 13/06/2024 07:29:54

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Methodological variations in the isolation of genomic DNA from Streptococcus bacteria	
Autores: 	Moreira,Mônica; Noschang,Juliana; Neiva,Ivana Froede; Carvalho,Yanê; Higuti,llma Hiroko; Vicente,Vânia Aparecida.	
Data: 	2010-08-01	
Ano: 	2010	
Palavras-chave: 	Streptococcus; DNA isolation; CTAB; Ultrasound.	
Resumo: 	In this work, genomic DNA of Streptococcus pyogenes, S. mutans and S. sobrinus was isolated using two methods: either using the detergent cetyltrimethylammonium bromide (CTAB) at 65ºC; or by applying ultrasound to a mixture of silica and celite in CTAB. The composite method that used ultrasound was the more efficient, allowing the straightforward extraction of genomic DNA from Gram-positive bacteria with good quality and reproducibility.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400013	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132010000400013	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.53 n.4 2010	
Fonte: 	info:eu-repo/semantics/openAccess