Data/hora: 03/06/2024 03:36:23

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety	
Autores: 	Tan,Dao Xuan; Tuong,Ho Manh; Thuy,Vu Thi Thu; Son,Le Van; Mau,Chu Hoang.	
Data: 	2015-10-01	
Ano: 	2015	
Palavras-chave: 	Drought-resistant; GmDREB2 gene; Gene transfer; Soybean; Transcription factors.	
Resumo: 	ABSTRACTThis work studied the amplification, cloning and determination of the GmDREB2 gene from the soybean cultivar DT2008 and five Vietnamese local soybean cultivars (DT26, DT51, DVN5, CB, CBD) and designed the vector carrying the structure containing GmDREB2 gene from cultivar DT2008 (best drought tolerant). The coding region of GmDREB2 gene isolated from six soybean cultivars was 480 nucleotides in length, encoding 159 amino acids. The recombinant structure was designed as 35S- GmDREB2- c-myc and its expression was analysed in transgenic tobacco plants. Recombinant DREB2 protein was expressed in five transgenic tobacco lines with molecular weights close to 20 kDa. During the drought conditions, the proline accumulation of the transgenic tobacco lines was higher than on wild-type (WT) plants, with the rate from 211.17 to 332.44% after five days of drought stress, and from 262.79 to 466.04% after nine days of drought stress. The two lines, TG2 and TG12 had the highest increase rate. These results provided the basis to generate drought-tolerant soybean plants by GmDREB2 overexpression.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500651	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132015050170	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.58 n.5 2015	
Fonte: 	info:eu-repo/semantics/openAccess