Data/hora: 05/06/2024 18:19:51

Provedor de dados: | 52
País: | Brazil
Título: | Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents
Autores: | Rodrigues,Marcelo; Festucci-Buselli,Reginaldo Alves; Silva,Luzimar Campos; Otoni,Wagner Campos.
Data: | 2014-04-01
Ano: | 2014
Palavras-chave: | Neem; HPLC; Glucose; Hydrolysed casein; Methyl jasmonate.
Resumo: | The use of cell and plant tissues culture techniques to produce economically important active metabolites has been growing. Among these substances, azadirachtin (AZA), produced by the neem tree (Azadirachta indica), has received considerable attention due to its bioinsecticide action. The main goal of this work was to analyze the AZA levels in neem cotyledonary calli. The calli were grown in agitated Woody Plant Medium (WPM) liquid medium, supplemented with glucose (Gl), hydrolyzed casein (HC) and methyl jasmonate (MeJ) as elicitor agent. An interaction was observed between these substances, depending on in vitro cultivation time with orbital agitation. The highest concentrations (average of 0.2470 µg g-1) of AZA were produced in the first and second weeks of culture when the cell mass was grown in a medium with 2% Gl v/v, 500 mg L-1 HC and 100 µM of MeJ. This corresponded to approximately 57% of the AZA content stored in the donor plants seeds, used as a source of explants to induce in vitro callus formation. It was concluded that the nutrition, as well as the concentration of MeJ as signal transduction of secondary metabolism in neem cells, might influence the AZA content produced in vitro.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200001
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132014000200001
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.57 n.2 2014
Fonte: | info:eu-repo/semantics/openAccess