Data/hora: 01/06/2024 01:23:34

Provedor de dados: | 52
País: | Brazil
Título: | Use of chitosan gel for the purification of protein
Autores: | Tang,ZhenXing; Qian,JunQing.
Data: | 2007-03-01
Ano: | 2007
Palavras-chave: | Chitosan; Gel; Preparation; Application.
Resumo: | The aim of this study was to optimize the preparation of chitosan gel and to use it for protein purification. The optimized preparation parameters were chitosan concentration 2.0%, glutaraldehyde concentration 0.6%, low cross-linking rate, NaOH concentration 1.6%, amount of NaBH4 0.4 g. In order to use the chitosan gel, the elution conditions were optimized as follows. NaCl concentration 0.05 mol/L in the tris-HCl (pH 9.05) at the flow rate of 2.03.0 mL/min. Particle size of chitosan gel was 120-140 µm. Neutral protease could be separated into four ingredients through chitosan gel column. The yield of enzyme was more than 90%. Albumin bovine serum could be separated into two ingredients through gel column and the total yield of albumin bovine serum was more than 70%.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200015
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132007000200015
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.50 n.2 2007
Fonte: | info:eu-repo/semantics/openAccess