Data/hora: 05/06/2024 16:13:03

Provedor de dados: | 52
País: | Brazil
Título: | Effects of chronic sucralose sweetener on the labeling of blood constituents with technetium-99m, morphology of red blood cells and the biodistribution of sodium pertechnetate in rats
Autores: | Rocha,Gabrielle de Souza; Pereira,Marcia de Oliveira; Benarroz,Mônica de Oliveira; Frydman,Jacques Natan Grinapel; Garcia-Pinto,Angélica Beatriz; Pereira,Mário José; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
Data: | 2008-12-01
Ano: | 2008
Palavras-chave: | Biodistribution; Blood; Morphology; Sweetener; Technetium-99m.
Resumo: | This work evaluates effects of the sweetener with sucralose on the labeling of blood constituents with technetium-99m (99mTc), on the morphology of red blood cells (RBC) and on the biodistribution of sodium pertechnetate in Wistar rats. Animals were treated with sweetener for 8 days. Blood samples were withdrawn and the assay of labeling of blood constituents with 99mTc was performed. Blood cells (BC) and plasma (P) were isolated. Aliquots of BC and P were also precipitated, soluble and insoluble fractions separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the RBC was evaluated under optical microscopy. In biodistribution experiments, sodium pertechnetate was administrated, organs and tissues isolated, radioactivity was counted and percentage of incorporated radioactivity per gram (%ATI/g) determined. The data showed no significant alterations in %ATI, morphology of RBC and in %ATI/g in the studied organs.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700021
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132008000700021
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.51 n.spe 2008
Fonte: | info:eu-repo/semantics/openAccess