Data/hora: 09/06/2024 13:28:57

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Variation of Hydroxyapatite Content in Soft Gelatin Affects Mesenchymal Stem Cell Differentiation	
Autores: 	Kantawong,Fahsai; Tanum,Junijira; Wattanutchariya,Wassanai; Sooksaen,Pat.	
Data: 	2016-01-01	
Ano: 	2016	
Palavras-chave: 	Gelatin; Hydroxyapatite; Mesenchymal stem cells; Gene Expression.	
Resumo: 	Gelatin is a common material used in tissue engineering and hydroxyapatite (HA) has a composition and structure similar to natural bone mineral. HA is also used to increase the adhesion ability of scaffolds. The physical and mechanical properties of gelatin, together with the chemical properties of HA, can affect cell differentiation. The main purpose of this study is to investigate the gene expression of human mesenchymal stem cells (HMSCs) upon culturing on gelatin composite with HA. Low amounts of HA were introduced into the gelatin in order to modulate properties of gelatin. Three types of hydrogel were fabricated by glutaraldehyde crosslinking before lyophilization to produce the porous 3D structure: (1) pure gelatin, (2) 0.5 mg/ml HA in gelatin, and (3) 1 mg/ml HA in gelatin. The fabricated hydrogels were used as scaffolds to cultivate HMSCs for two periods - 24 hours and 3 weeks. The results showed that all types of fabricated hydrogels could be used to cultivate HMSCs. Changes of gene expressions indicated that the HMSCs cultured on the 1 mg/ml HA in gelatin showed neuronal lineage-specific differentiation.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100411	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/1678-4324-2016150650	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.59 2016	
Fonte: 	info:eu-repo/semantics/openAccess