Data/hora: 01/06/2024 00:57:44

Provedor de dados: | 52
País: | Brazil
Título: | A Novel Raw Starch Hydrolyzing Thermostable α-Amylase Produced by Newly Isolated Bacillus mojavensis SO-10: Purification, Characterization and Usage in Starch Industries
Autores: | Ozdemir,Sadin; Fincan,Sema Aguloglu; Karakaya,Adem; Enez,Baris.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | Α-Amylase; Raw starch; Thin-Layer Chromatography (TLC); Detergents; Apple Juice Clarification.
Resumo: | ABSTRACT The aim of this study is the production, purification, and characterisation of thermostable raw starch hydrolyzing α-amylase produced by Bacillus mojavensis SO-10. The maximum production conditions of α-amylase were found at 36th hour, 35 °C and pH 7.0. We utilized three steps to purify the thermostable α-amylase and as a result, 34-fold and 18% yield were obtained. The molecular weight of purified α-amylase was determined as 73 kD. The Km and Vmax rates were detected as 0.010 mM and 3.38 µmol min−1, respectively. This purified α-amylase exhibited the highest activity at pH 5.0-6.0 and 70 ºC and showed stability over a wide variety of pH and temperature at 4.0-8.0, and 40-50 ºC, respectively. The thermostable purified α-amylase exhibited stability in the presence of denaturing agents and heavy metal ions. The purified enzyme hydrolyzed the raw starches of corn and wheat grains in the ratio of 36.7% and 39.2% respectively. The end-yields of soluble starch hydrolysis were analyzed by thin-layer chromatography (TLC). In addition, the usage of purified α-amylase in clarification of apple juice and domestic washing detergent industries were evaluated.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100412
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/1678-4324-2018160399
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.61 2018
Fonte: | info:eu-repo/semantics/openAccess