Data/hora: 08/06/2024 14:21:13

Provedor de dados: | 52
País: | Brazil
Título: | Plant regeneration and Agrobacterium-mediated transformation of Achyranthes bidentata using cotton EREBP gene
Autores: | Duan,Hong Ying; Ding,Xiao Sheng; Song,Jian Ying; He,Yun Long; Zhou,Yan Qing.
Data: | 2013-06-01
Ano: | 2013
Palavras-chave: | Achyranthes bidentata; EREBP gene; Agrobacterium tumefaciens; Genetic transformation.
Resumo: | The aim of this work was to study the plant regeneration and Agrobacterium-mediated transformation of Achyranthes bidentata using cotton EREBP gene. Results showed that the callus induction rate of stems from A. bidentata was the highest (100%) and bud was in approximately 70% of calli from stems. However bud differentiation rate of the callus from leaves and petioles was very low. Compared with ceftriaxone, 200mg/L cefotaxime could completely control Agrobacterium tumefaciens and had relatively less toxic action on the stems of A. bidentata. In addition, the induction rate of callus resistant to hygromycin was the highest when infected for 3 min and co-cultivated for 3 d. Six positive transgenic plants transformed with pCAMBIA1304-GhEREB2 expression vector were obtained and confirmed by PCR. The expression of target gene GhEREB2 was detected in five transgenic plants by RT-PCR. In brief, an efficient system of genetic transformation and plant regeneration was established for A. bidentata.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300001
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132013000300001
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.56 n.3 2013
Fonte: | info:eu-repo/semantics/openAccess