Data/hora: 05/06/2024 09:59:33

Provedor de dados: | 52
País: | Brazil
Título: | Light microscope observation of circulating human lymphocytes cultured in vitro
Autores: | Oliveira,Naila Francis Paulo de; Dolder,Mary Anne Heidi; Genari,Selma Candelária.
Data: | 2010-10-01
Ano: | 2010
Palavras-chave: | Blood; In vitro; Lymphocytes; Microscopy; Morphology; Percoll.
Resumo: | The purpose of this work was to study the isolation and a light microscopy technique for cultured lymphocytes. Blood samples were obtained by venipuncture with an anticoagulant added and centrifuged in a Percoll density gradient to separate the leukocytes. Lymphocytes were placed in 25 cm ³ tissue culture flasks at 37ºC. After culturing, they were fixed and stained with the methods used for blood smears. Results showed that not all fixing solutions and stains were an equally good choice for cultured lymphocytes.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500013
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132010000500013
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.53 n.5 2010
Fonte: | info:eu-repo/semantics/openAccess