Data/hora: 05/06/2024 09:11:45

Provedor de dados: | 52
País: | Brazil
Título: | Characterization of an alcoholic hepatic steatosis model induced by ethanol and high-fat diet in rats
Autores: | Souza,Carlos Eduardo Alves de; Stolf,Aline Maria; Dreifuss,Arturo Alejandro; Lívero,Francislaine dos Reis; Gomes,Liana de Oliveira; Petiz,Lyvia; Beltrame,Olair; Dittrich,Rosangela Locatelli; Telles,José Ederaldo Queiroz; Cadena,Sílvia Maria; Acco,Alexandra.
Data: | 2015-06-01
Ano: | 2015
Palavras-chave: | Ethanol; Alcoholic hepatic steatosis; High-fat diet; Sunflower seeds; Mitochondria.
Resumo: | Alcoholic liver disease is characterized by a wide spectrum of liver damage, which increases when ethanol is associated with high-fat diets (HFD). This work aimed to establish a model of alcoholic hepatic steatosis (AHS) by using a combination of 10% ethanol and sunflower seeds as the source of HFD. Male rats received water or 10% ethanol and regular chow diet and/or HFD, which consisted of sunflower seeds. The food consumption, liquid intake and body weight of the rats were monitored for 30 days. After this period, blood was collected for biochemical evaluation, and liver samples were collected for histological, mitochondrial enzyme activity and oxidative stress analyses. Our results indicated that the combination of 10% ethanol and HFD induced micro- and macrosteatosis and hepatocyte tumefaction, decreased the levels of reduced glutathione and glutathione S-transferase activity and increased the level of lipoperoxidation and superoxide dismutase activity. The mitochondrial oxidation of NADH and succinate were partially inhibited. Complexes I and II were the main inhibition sites. Hepatic steatosis was successfully induced after 4 weeks of the diet, and the liver function was modified. The combination of 10% ethanol and sunflower seeds as an HFD produced an inexpensive model to study AHS in rats.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300367
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-8913201500294
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.58 n.3 2015
Fonte: | info:eu-repo/semantics/openAccess