Data/hora: 06/06/2024 10:29:44

Provedor de dados: | 52
País: | Brazil
Título: | Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m
Autores: | Moreno,Silvana Ramos Farias; Carvalho,Jorge José de; Nascimento,Ana Lúcia; Olej,Beni; Rocha,Emely Kazan; Arnobio,Adriano; Bernardo-Filho,Mário; Caldas,Luiz Querino de Araújo; Honeycut,Hayden.
Data: | 2008-12-01
Ano: | 2008
Palavras-chave: | Uncaria tomentosa; Technetium-99m; Radiolabeling; Blood constituents.
Resumo: | The influence (in vivo and in vitro) of an Uncaria tomentosa extract (Cats claw) on the labeling of red blood cells (RBCs) and plasma and cellular proteins with technetium-99m (Tc-99m) was evaluated. For the in vivo treatment, animals were treated with Cats claw. For the in vitro treatment, heparinized blood was incubated with Cats claw before the addition of stannous chloride (SnCl2) and Tc-99m. Samples of plasma (P) and RBCs were separated and also precipitated with trichloroacetic acid. The soluble and insoluble fractions of P and RBCs were isolated. The analysis of the results of the in vivo study, indicates that there is no significant alteration on the uptake of Tc-99m by the blood constituents, but it significantly decrease (p&lt;0.05) the labeling of blood constituents by in vitro methods. These effects could be due to chelation of stannous and /or pertechnetate ions and blockage of the Tc-99m bindings sites.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700024
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132008000700024
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.51 n.spe 2008
Fonte: | info:eu-repo/semantics/openAccess