Data/hora: 06/06/2024 02:13:22

Provedor de dados: | 52
País: | Brazil
Título: | Characteristics of Immobilized Urease on Grafted Alginate Bead Systems
Autores: | Danial,Enas N.; Hamza,Amal H.; Mahmoud,Rasha H.
Data: | 2015-04-01
Ano: | 2015
Palavras-chave: | Urease; Immobilization; Kinetic; Reusability; Storage stability.
Resumo: | This study evaluated the biological importance of immobilized urease enzyme over the free urease. The support material used for urease immobilization was alginate. Generally, the immobilization of urease in alginate gel showed a marked increase in Km and Vmax. However, the immobilized urease showed higher thermal stability than that of free enzyme. The rate of thermal inactivation of the immobilized enzyme decreased due to entrapment in gel matrix. Also, the activity of the immobilized urease was more stable in retention than that of the free enzyme during the storage in solution, although the activity of the immobilized enzyme was lower in comparison with the free enzyme. A stable immobilized system and long storage life are convenient for applications that would not be feasible with a soluble enzyme system. These results highlighted the technical and biochemical benefits of immobilized urease over the free enzyme.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200147
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-8913201400204
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.58 n.2 2015
Fonte: | info:eu-repo/semantics/openAccess