Data/hora: 09/06/2024 04:12:24

Provedor de dados: | 52
País: | Brazil
Título: | Antitumor activity of C-phycocyanin from Arthronema africanum (Cyanophyceae)
Autores: | Gardeva,Elena; Toshkova,Reneta; Yossifova,Liliya; Minkova,Kaledona; Ivanova,Natalia; Gigova,Liliana.
Data: | 2014-10-01
Ano: | 2014
Palavras-chave: | Antitumor activity; Arthronema africanum; In vivo; In vitro; C-pycocyanin; Myeloid Graffi tumor.
Resumo: | Pure C-phycocyanin (C-PC) was isolated from Arthronema africanumto evaluate its potential antitumor effects in vivo and in vitro. Experimental myeloid Graffi tumor in hamsters was used as a model. The cell proliferation assay showed that C-PC treatment, at concentration of 100 µg mL-1 for 24 h, significantly inhibited the growth of Graffi tumor cells (51.4% viability). Agarose gel electrophoresis of the genomic DNA of treated cells displayed time-and concentration-dependent fragmentation pattern, typical for apoptosis. Apoptotic process was related to the increase in cellular manganese and copper/zinc superoxide dismutases and glutathione reductase activities, coupled with a low catalase activity. In vivo C-PC administration (5.0 mg kg-1 body weight) suppressed the tumor transplantability and growth, while the mean survival time of the tumor-bearing hamsters was increased. The results revealed promising antitumor activities of A. africanum C-PC and suggested the potential of this natural biliprotein pigment for future pharmacological and medical applications. The study provided new data on the mechanism of the C-PC induced apoptosis in which the imbalance of antioxidant enzymes that favoured hydrogen peroxide accumulation might play a leading role.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500675
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132014005000018
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.57 n.5 2014
Fonte: | info:eu-repo/semantics/openAccess