Data/hora: 07/06/2024 05:49:23

Provedor de dados: | 52
País: | Brazil
Título: | Rice bran as a substrate for proteolytic enzyme production
Autores: | Sumantha,Alagarsamy; Deepa,Paul; Sandhya,Chandran; Szakacs,George; Soccol,Carlos Ricardo; Pandey,Ashok.
Data: | 2006-09-01
Ano: | 2006
Palavras-chave: | Solid-state fermentation; Rice bran; Rhizopus; Neutral protease; Agro-industrial residues.
Resumo: | Rice bran was used as the substrate for screening nine strains of Rhizopus sp. for neutral protease production by solid-state fermentation. The best producer, Rhizopus microsporus NRRL 3671, was used for optimizing the process parameters for enzyme production. Fermentation carried out with 44.44 % initial moisture content at a temperature of 30 C for 72 h was found to be the optimum for enzyme secretion by the fermenting organism. While most of the carbon supplements favored enzyme production, addition of casein resulted in a marginal increase in protease yield. Fermentation was then carried out under optimized conditions to obtain the crude extract of the enzyme, which was partially purified by precipitation and dialysis. A 3-fold increase in the enzyme purity was achieved in this manner. The enzyme was found to be a metalloprotease, being activated by Mn2+, with maximal activity at a temperature of 60 C and pH 7.0.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600019
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132006000600019
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.49 n.5 2006
Fonte: | info:eu-repo/semantics/openAccess