Data/hora: 07/06/2024 01:04:59

Provedor de dados: | 54
País: | Brazil
Título: | Genotoxic activity of the insecticide Nuvacron (Monocrotophos) detected by the micronucleus test in bone marrow erythrocytes of mice and in CHO cells
Autores: | Peitl Jr,Paulo; Sakamoto-Nojo,Elza T.; Cólus,Ilce M. de Syllos.
Data: | 1996-01-01
Ano: | 1996
Palavras-chave: | Genotoxic; Insecticide Nuvacron; Micronucleus; Bone marrow; Erythrocytes; Mice; CHO cells.
Resumo: | The organophosphorus insecticide Nuvacron (Monocrotophos) is a very toxic agent widely utilized in Brazilian agriculture. To evaluate the clastogenic potential of this insecticide, in vivo and in vitro micronucleus (MN) assay experiments were carried out on Swiss mice and on Chinese hamster ovary (CHO) cells, respectively. Nuvacron administered at doses of 2.5 and 5.0 mg/kg induced a statistically significant increase in the frequencies of MN detected in polychromatic bone marrow erythrocytes from animals (six/group) treated ip 24 h before. Exponentially growing CHAO cells were treated continuously (16h) with Nuvacron diluted in water to final concentrations of 1, 10, 100, 200, and 400 mug/ml. Three experiments were carried out using the cytokinesis-block method and a total of 6000 binucleated cells were scored to determine MN frequencies. A statistically significant increase in the frequencies of MN was observed for the cells treated with 1 and 10 mug/ ml Nuvacron. A marked decrease in cell proliferation rates was observed for CHO cultures treated with higher concentrations. These data demonstrate that Nuvacron has a genotoxic effect on both in vivo and in vitro mammalian test systems.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400006
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S0100-84551996000400006
Formato: | text/html
Fonte: | Brazilian Journal of Genetics  v.19 n.4 1996
Fonte: | info:eu-repo/semantics/openAccess