Data/hora: 15/06/2024 15:52:23

Provedor de dados: | 55
País: | Brazil
Título: | Structure-function analysis of two variants of mumps virus hemagglutinin-neuraminidase protein
Autores: | Santos-López,Gerardo; Scior,Thomas; Borraz-Argüello,María del Tránsito; Vallejo-Ruiz,Verónica; Herrera-Camacho,Irma; Tapia-Ramírez,José; Reyes-Leyva,Julio.
Data: | 2009-02-01
Ano: | 2009
Palavras-chave: | Hemagglutinin-neuraminidasa; Mumps virus; Paramyxovirus; 3D structure prediction; Sialic acid.
Resumo: | A point mutation from guanine (G) to adenine (A) at nucleotide position 1081 in the hemagglutinin-neuraminidase (HN) gene has been associated with neurovirulence of Urabe AM9 mumps virus vaccine. This mutation corresponds to a glutamic acid (E) to lysine (K) change at position 335 in the HN glycoprotein. We have experimentally demonstrated that two variants of Urabe AM9 strain (HN-A1081 and HN-G1081) differ in neurotropism, sialic acidbinding affinity and neuraminidase activity. In the present study, we performed a structure-function analysis of that amino acid substitution; the structures of HN protein of both Urabe AM9 strain variants were predicted. Based on our analysis, the E/K mutation changes the protein surface properties and to a lesser extent their conformations, which in turn reflects in activity changes. Our modeling results suggest that this E/K interchange does not affect the structure of the sialic acid binding motif; however, the electrostatic surface differs drastically due to an exposed short alpha helix. Consequently, this mutation may affect the accessibility of HN to substrates and membrane receptors of the host cells. Our findings appear to explain the observed differences in neurotropism of these vaccine strains.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100007
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1590/S1413-86702009000100007
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.13 n.1 2009
Fonte: | info:eu-repo/semantics/openAccess