Data/hora: 11/06/2024 04:14:29

Provedor de dados: | 55
País: | Brazil
Título: | Evaluation of phenotypic tests to detect carbapenem-resistant Enterobacteriaceae in colonized patients hospitalized in intensive care units
Autores: | Perez,Leandro Reus Rodrigues; Rodrigues,Diógenes; Dias,Cícero Gomes.
Data: | 2015-08-01
Ano: | 2015
Palavras-chave: | Carbapenems; Enterobacteriaceae; KPC; Surveillance.
Resumo: | In this study, we aimed to evaluate the performance of different phenotypic tests to detect carbapenem-resistant Enterobacteriaceae. Three different phenotypic methods were evaluated: (1) combined-disk test of meropenem plus phenylboronic acid or EDTA reading after 24 h and 48 h; (2) selective/chromogenic read after 24 h and after 48 h; and (3) overnight selective enrichment broth containing 10 µg ertapenem disk followed by culture on MacConkey agar. A positive result in at least one of the methods was submitted to PCR for blaNDM-1, blaOXA-48, blaKPC, blaSPM-1, blaIMP, and blaGES detection. Carbapenem-resistant Enterobacteriaceae was detected in 31 (30.4%) of 102 rectal swabs evaluated. All isolates showed to be KPC-2-producing organisms. Results showed excellent agreement among the evaluated tests (positive and negative) (kappa = 0.88). It is important to state that combined-disk test with phenylboronic acid is not suitable for bacterial identiﬁcation/isolation. Conversely, selective/chromogenic agar after 48h of incubation showed to be a useful tool, with the advantage of presumptive bacterial identiﬁcation.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000400436
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1016/j.bjid.2015.03.008
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.19 n.4 2015
Fonte: | info:eu-repo/semantics/openAccess