Data/hora: 13/06/2024 21:51:17

Provedor de dados: 	55	
País: 	Brazil	
Título: 	Prevalence of resistance-associated mutations in Human Immunodeficiency Virus type 1-positive individuals failing HAART in Rio de Janeiro, Brazil	
Autores: 	Varella,Rafael Brandão; Ferreira,Selma Baía; Castro,Márcia Braga de; Tavares,Marisa Dias; Zalis,Mariano Gustavo.	
Data: 	2008-10-01	
Ano: 	2008	
Palavras-chave: 	HIV-1; HAART; Reverse transcriptase; Protease; Resistance.	
Resumo: 	We investigated the occurrence of HIV-1 antiretroviral resistance in individuals failing to respond to highly active antiretroviral therapy (HAART) attended by RENAGENO from 2001-2004. One hundred and seventeen patients were selected for this study; their plasma viral RNA was extracted and the PR and RT genes sequenced to examine subtype, genetic polymorphisms and mutations associated with resistance to antiretroviral drugs. HIV-1 sequence analysis showed that 86/100 (86%) were infected with subtype B, 7/100 (7%) with subtype F and 7/100 (7%) with RT/PR hybrid forms (2 D/B, 2 F/B, 2 B/F and 1 D/F). In 14 (12%) of the samples, the subtype was not determined. The prevalence of resistance mutations was high (93.1%), mainly in the RT gene. The most prevalent resistance mutations were: M184V (60.7%), T215Y (49.6%) and M41L (46.7%) in the RT gene and L90M (19.6%), M46I (16.2%) and D30N (12.8%) in the PR gene. The frequency of resistance mutations tended to increase from the first to the second therapeutic scheme failure (p=0.079); but it stabilized after subsequent failures (p=0.875). Our finding of a high frequency of drug resistant HIV-1 samples supports the need for continuous genotypic monitoring of patients failing HAART.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500007	
Editor: 	Brazilian Society of Infectious Diseases	
Relação: 	10.1590/S1413-86702008000500007	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Infectious Diseases  v.12 n.5 2008	
Fonte: 	info:eu-repo/semantics/openAccess