Data/hora: 01/06/2024 09:53:17

Provedor de dados: 	55	
País: 	Brazil	
Título: 	Molecular epidemiology of heteroresistant vancomycin-intermediate Staphylococcus aureus in Brazil	
Autores: 	Silveira,Alessandro Conrado de Oliveira; Cunha,Gabriela Rosa da; Caierão,Juliana; Cordova,Caio Mauricio Mendes de; d'Azevedo,Pedro Alves.	
Data: 	2015-10-01	
Ano: 	2015	
Palavras-chave: 	MRSA; Small colony variants; Antimicrobial resistance.	
Resumo: 	ABSTRACTTo determine the epidemiological and molecular characteristics of 12 Staphylococcus aureus isolates presenting heteroresistance to vancomycin in laboratories of two cities in Santa Catarina, southern Brazil. Epidemiological data, including the city of isolation, health institution, and date of isolation were considered, as well as the associated clinical specimen. For molecular characterization, we analyzed the staphylococcal cassette chromosome types, the erm gene presence, and the genomic diversity of isolates using pulsed-field gel electrophoresis. The 12 isolates of S. aureus were previously confirmed as heteroresistance to vancomycin using the population analysis profile-area under curve. Regarding genetic variability, two clones were detected: the main one (clone A) composed of four isolates and the clones B, with two isolates. For clone A, two isolates presented identical band patterns and were related to the same hospital, with an interval of 57 days between their isolation. The other isolates of this clone showed no epidemiological link between them because they were isolated in different hospitals and had no temporal relationship. The other clone showed no detectable epidemiological relationship. The heteroresistance to vancomycin recovered in Santa Catarina State from 2009 to 2012 had, in general, heterogeneous genomic patterns based on pulsed-field gel electrophoresis results, which is in accordance with the fact that these isolates had little or no epidemiological relationship among them. Due to the characteristic phenotypic instability and often prolonged vancomycin therapy for selection, clonal spread is not as common as for other resistance mechanisms disseminated through horizontal gene transfer.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000500466	
Editor: 	Brazilian Society of Infectious Diseases	
Relação: 	10.1016/j.bjid.2015.06.013	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Infectious Diseases  v.19 n.5 2015	
Fonte: 	info:eu-repo/semantics/openAccess