Data/hora: 01/06/2024 10:28:01

Provedor de dados: | 55
País: | Brazil
Título: | Molecular characterization of microbial contaminants isolated from Umbilical Cord Blood Units for transplant
Autores: | Bello-López,Juan Manuel; Noguerón-Silva,Jorge; Castañeda-Sánchez,Jorge Ismael; Rojo-Medina,Julieta.
Data: | 2015-12-01
Ano: | 2015
Palavras-chave: | Umbilical Cord Blood Units; Microbial contamination; Molecular characterization.
Resumo: | ABSTRACT Disposal of Umbilical Cord Blood Units due to microbial contamination is a major problem in Cord Blood Banks worldwide as it reduces the number of units available for transplantation. Additionally, economic losses are generated as result of resources and infrastructure used to obtain such units. Umbilical Cord Blood Units that showed initial microbial contamination were subject to strains isolation, identification, and characterization by sequencing the 16S rRNA gene and Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR). Moreover, tests of antimicrobial resistance/sensitivity and phenotypic activities that may play an important role in microbial infection were performed. Microbial contamination was detected in 120 Umbilical Cord Blood Units (2.31%) in the period from 2003 to 2013. The most frequently isolated strains were Enterococcus faecium, followed by Staphylococcus epidermidis, Escherichia coli, Enterococcus faecalis, Staphylococcus haemoliticus, Klebsiella pneumoniae, Enterococcus durans, Lactobacillus helveticus, Enterococcus hiriae and Roseomonas genomospecies 5. The ERIC-PCR assays revealed a wide genetic diversity in some strains although belonging to the same genus and specie, indicating different sources of contamination. Broad-spectrum penicillins, third generation cephalosporins, aminoglycosides, and fluoroquinolones showed lower inhibitory activity on the tested strains. All strains were proteolytic, 67.69% were amylase-positive, 27.6% hemolysis-positive, and 34.71% nuclease-positive. The most common sources of contamination were: vaginal flora, digestive tract, and skin flora, highlighting the need for staff training in good manufacturing practices in collection SCU since all contaminants identified are part of the microbial flora of the donors. Implications and consequences in the therapeutic use of Umbilical Cord Blood Units for transplantation contaminated by multiresistant bacteria in immunocompromised patients are discussed.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600571
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1016/j.bjid.2015.07.005
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.19 n.6 2015
Fonte: | info:eu-repo/semantics/openAccess