Data/hora: 05/06/2024 19:12:57

Provedor de dados: | 55
País: | Brazil
Título: | Comparison of different primes for PCR-based diagnosis of cutaneous leishmaniasis
Autores: | Oliveira,Diego Molina de; Lonardoni,Maria Valdrinez Campana; Teodoro,Ueslei; Silveira,Thais Gomes Verzignassi.
Data: | 2011-06-01
Ano: | 2011
Palavras-chave: | Leishmaniasis; Polymerase chain reaction; Leishmania; DNA primers.
Resumo: | OBJECTIVE: The objective of this study was to analyze different primers that are commonly used in epidemiological studies for the detection of Leishmania DNA by PCR, and to compare them to the conventional direct parasite search for American cutaneous leishmaniasis (ACL) diagnosis. MATERIAL AND METHODS: Five pairs of primers, four of them derived from Leishmania kDNA sequences (MP3H-MP1L; B1-B2; LBF1-LBR1; 13A-13B), and one derived from the SL RNA (mini-exon) gene repeat (LU5A-LB3C), reported previously, were used. RESULTS: The MP3H-MP1L primers were the best at amplifying the DNA, detecting 2 fg of Leishmania spp. DNA. The 13A-13B primers presented the worst performance, detecting 512 x 10³ fg of DNA. CONCLUSION: The wide variation in the analytical sensitivity of the primers used in the PCR, and the significant differences from the conventional method of ACL diagnosis found in this study, emphasize the importance of standardizing the PCR technique, analyzing sensitivity, and selecting suitable oligonucleotide primers.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300004
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1590/S1413-86702011000300004
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.15 n.3 2011
Fonte: | info:eu-repo/semantics/openAccess