Data/hora: 14/06/2024 02:27:34

Provedor de dados: | 55
País: | Brazil
Título: | Association between the p53 arginine/arginine homozygous genotype at codon 72 and human papillomavirus E6/E7 mRNA expression
Autores: | Chuery,Ana Carolina Silva; Silva,Ismael Dale Cotrim Guerreiro da; Ribalta,Julisa Chamorro Lascasas; Speck,Neila Maria de Góis.
Data: | 2017-06-01
Ano: | 2017
Palavras-chave: | Human papillomavirus; Oncoproteins; Genetic polymorphisms; P53 gene; Arginine.
Resumo: | ABSTRACT  Objective: To evaluate the association between p53 polymorphisms and human papillomavirus (HPV) E6/E7 mRNA expression.  Methods: We analyzed 175 cervical samples from women aged 16-69 years old who were tested for HPV E6/E7 mRNA expression (NucliSENS® EasyQ® HPV). The samples were divided into three groups: positive (n = 75) those with positive HPV E6/E7 mRNA expression and positive high-risk HPV Hybrid Capture (HR-HC) test; negative (n = 52) those with negative HPV E6/E7 mRNA expression and positive HR-HC; and control (n = 48) those with negative HPV E6/E7 mRNA expression and negative HR-HC. The p53 polymorphisms at codons 11, 72, and 248 were evaluated through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).  Results: The frequency of the arginine/arginine homozygous genotype at codon 72 was significantly higher in the positive (49.3%) than in the negative (32.7%) and control groups (20.8%, p = 0.002*). The frequency of the arginine allele was also significantly higher in the positive (67.3%) than in the negative (53.8%) and control groups (38.5%, p &lt; 0.001*). The arginine/arginine homozygous genotype was significantly associated with positive HPV E6/E7 mRNA expression (positive group) compared with negative and control groups (odds ratio: 2.633; 95% CI, 1.399-4.954, p = 0.003). The frequency of arginine/arginine homozygous genotype at codon 72 remained significantly more frequent in the positive group of women aged ≥30 years than in the other two groups.  Conclusion: The presence of the p53 arginine/arginine homozygous genotype at codon 72 was significantly associated with the positive HPV E6/E7 mRNA expression.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300248
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1016/j.bjid.2017.03.002
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.21 n.3 2017
Fonte: | info:eu-repo/semantics/openAccess