Data/hora: 11/06/2024 11:00:01

Provedor de dados: | 55
País: | Brazil
Título: | Identification and characterization of previously described epitopes in HIV-1 subtypes B, C, F and BF in Brazil
Autores: | Queiroz,Artur Trancoso Lopo de; Santos,Luciane Amorim; Moreau,Domingos Ramon; Oliveira,Tulio de; Watkins,David I.; Galvão-Castro,Bernardo; Alcantara,Luiz Carlos Junior.
Data: | 2007-02-01
Ano: | 2007
Palavras-chave: | HIV-1; Env; Vaccine; Epitope.
Resumo: | Genetic analysis of HIV-1 is essential to improve treatment strategies and select epitopes for vaccine programs. The objective of this study was to determine whether known CD4+ and CD8+ epitopes were present in Brazilian HIV-1 strains. We used previously described CD8+ and CD4+ epitopes from the Los Alamos laboratory to search for these epitopes in the Brazilian sequences using the HIVbase program and we compared the frequency results with the analyses using physical-chemical profile tools from Network Protein Sequence Analysis (NPSA), and the SYFPEITHI program. Furthermore, this analysis was carried out with the Prosite tool using the GeneDoc program and ds/dn analyses using the Synonymous Nonsynonymous Analysis Program (SNAP). The HIVbase epitope mapping demonstrated that 30 CD8+ and 6 CD4+ epitopes were present in the Brazilian sequences at a high frequency. Only two of these epitopes were heavily glycosylated. Interestingly, ds/dn analyses showed evidence of purifying selective pressure. These types of analyses could be useful for the assessment of possible vaccine efficiency in populations.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100008
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1590/S1413-86702007000100008
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.11 n.1 2007
Fonte: | info:eu-repo/semantics/openAccess