Data/hora: 10/06/2024 11:15:56

Provedor de dados: | 55
País: | Brazil
Título: | Simultaneous detection of hepatitis B virus genotypes and mutations associated with resistance to lamivudine, adefovir, and telbivudine by the polymerase chain reaction-ligase detection reaction
Autores: | Wang,Yong-Zhong; Xiao,Jun-Hua; Liu,Long-Gen; Ye,Chun-Yan; Shen,Hong-Yu; Xu,Tian-Min; Zhu,Ke-Zhuan.
Data: | 2011-12-01
Ano: | 2011
Palavras-chave: | Hepatitis B; Drug resistance; Mutation; Genotype.
Resumo: | OBJECTIVES: Detection of mutations associated to nucleos(t)ide analogs and hepatitis B virus (HBV) genotyping are essential for monitoring treatment of HBV infection. We developed a multiplex polymerase chain reaction-ligase detection reaction (PCR-LDR) assay for the rapid detection of HBV genotypes and mutations associated with lamivudine, adefovir, and telbivudine resistance in HBV-infected patients. METHODS: HBV templates were amplified by PCR, followed by LDR and electrophoresis on a sequencer. The assay was evaluated using plasmids that contained wild-type or mutant HBV sequences and 216 clinical samples. RESULTS: The PCR-LDR assay and sequencing gave comparable results for 158 of the 216 samples (73.1%) with respect to mutation detection and genotyping. Complete agreement between the two methods was observed for all the samples (100%) at codon 180 and codon 204. Concordant results were observed for 99.4% of the 158 samples at codon 181 and 98.7% at codon 236. The genotyping results were completely concordant between the PCR-LDR assay and sequencing. The PCR-LDR assay could detect a proportion of 1% mutant plasmid in a background of wild-type plasmid. CONCLUSION: The PCR-LDR assay is sensitive and specific for detection of HBV genotypes and drug resistance mutations, and could be helpful for decision making in the treatment of HBV infection.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000600010
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1590/S1413-86702011000600010
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.15 n.6 2011
Fonte: | info:eu-repo/semantics/openAccess