Data/hora: 07/06/2024 02:13:21

Provedor de dados: | 55
País: | Brazil
Título: | Effects of sub-minimum inhibitory concentrations of ciprofloxacin on biofilm formation and virulence factors of Escherichia coli
Autores: | Dong,Guofeng; Li,Jiahui; Chen,Lijiang; Bi,Wenzi; Zhang,Xiaoxiao; Liu,Haiyang; Zhi,Xiaoyang; Zhou,Tieli; Cao,Jianming.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | Biofilm formation; Ciprofloxacin; Escherichia coli; Sub-minimum inhibitory concentration; Virulence.
Resumo: | ABSTRACT  Objective: To evaluate the influence of sub-minimum inhibitory concentrations (MICs) of ciprofloxacin (CIP) on biofilm formation and virulence factors of Escherichia coli clinical isolates.  Methods: Sub-MICs of CIP were determined using growth curve experiments. The biofilm-forming capacity of E. coli clinical isolates and E. coli ATCC 25922 treated or untreated with sub-MICs of CIP was assessed using a crystal violet staining assay. The biofilm structure of E. coli isolate was assessed with scanning electron microscopy (SEM). The expression levels of the virulence genes fim, usp, and iron and the biofilm formation genes of the pgaABCD locus were measured using quantification RT-PCR (qRT-PCR) in E. coli isolates and E. coli ATCC 25922.  Results: Based on our results, the sub-MICs of CIP were 1/4 MICs. Sub-MICs of CIP significantly inhibited biofilm formation of E. coli clinical isolates and E. coli ATCC 25922 (p &lt; 0.01). SEM analyses indicated that the biofilm structure of the E. coli changed significantly after treatment with sub-MICs of CIP. Expression levels of the virulence genes fim, usp, and iron and the biofilm formation genes of the pgaABCD locus were also suppressed.  Conclusions: The results revealed that treatment with sub-MICs of CIP for 24 h inhibited biofilm formation and reduced the expression of virulence genes and biofilm formation genes in E. coli.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100015
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1016/j.bjid.2019.01.004
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.23 n.1 2019
Fonte: | info:eu-repo/semantics/openAccess