Data/hora: 10/06/2024 16:11:24

Provedor de dados: | 55
País: | Brazil
Título: | Validation and clinical application of a nested PCR for paracoccidioidomycosis diagnosis in clinical samples from Colombian patients
Autores: | Gaviria,Marcela; Rivera,Vanessa; Muñoz-Cadavid,Cesar; Cano,Luz Elena; Naranjo,Tonny Williams.
Data: | 2015-08-01
Ano: | 2015
Palavras-chave: | Paracoccidioides brasiliensis; Nested PCR; Paracoccidioidomycosis; Molecular diagnosis.
Resumo: | Paracoccidioidomycosis is a systemic and endemic mycosis, restricted to tropical and subtropical areas of Latin America. The infection is caused by the thermal dimorphic fungus Paracoccidioides brasiliensisand Paracoccidioides lutzii. The diagnosis of paracoccidioidomycosis is usually performed by microscopic examination, culture and immunodiagnostic tests to respiratory specimens, body fluids and/or biopsies; however these methods require laboratory personnel with experience and several days to produce a result. In the present study, we have validated and evaluated a nested PCR assay targeting the gene encoding the Paracoccidioides gp43membrane protein in 191 clinical samples: 115 samples from patients with proven infections other than paracoccidioidomycosis, 51 samples as negative controls, and 25 samples from patients diagnosed with paracoccidioidomycosis. Additionally, the speciﬁcity of the nested PCR assay was also evaluated using puriﬁed DNA isolated from cultures of different microorganisms (n= 35) previously identiﬁed by culture and/or sequencing. The results showed that in our hands, this nested PCR assay for gp43 protein showed speciﬁcity and sensitivity rates of 100%. The optimized nested PCR conditions in our laboratory allowed detection down to 1 fg of P. brasiliensisDNA.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000400376
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1016/j.bjid.2015.04.008
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.19 n.4 2015
Fonte: | info:eu-repo/semantics/openAccess