Data/hora: 09/06/2024 13:38:25

Provedor de dados: | 56
País: | Brazil
Título: | Mini-Mu insertions in the tetracycline resistance determinant from Proteus mirabilis
Data: | 1997-03-01
Ano: | 1997
Palavras-chave: | Tetracycline; Mutation; LacZ-fusion; Proteus mirabilis; Bacteriophage Mu.
Resumo: | The inducible tetracycline resistance determinant isolated from Proteus mirabilis cloned into the plasmid pACYC177 was mutagenized by insertion of a mini-Mu-lac phage in order to define the regions in the cloned sequences encoding the structural and regulatory proteins. Three different types of mutants were obtained: one lost the resistance phenotype and became Lac+; another expressed the resistance at lower levels and constitutively; the third was still dependent on induction but showed a lower minimal inhibitory concentration. The mutant phenotypes and the locations of the insertions indicate that the determinant is composed of a repressor gene and a structural gene which are not transcribed divergently as are other known tetracycline determinants isolated from Gram-negative bacteria
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300009
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1997000300009
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.30 n.3 1997
Fonte: | info:eu-repo/semantics/openAccess