Data/hora: 01/06/2024 01:22:46

Provedor de dados: | 56
País: | Brazil
Título: | Bovine serum albumin potentiates caffeine- or ATP-induced tension in human skinned skeletal muscle fibers
Data: | 1997-05-01
Ano: | 1997
Palavras-chave: | Calcium-release channel; Sarcoplasmic reticulum; Human muscle fiber; Bovine serum albumin; Caffeine.
Resumo: | Human skinned muscle fibers were used to investigate the effects of bovine serum albumin (BSA) on the tension/pCa relationship and on the functional properties of the Ca2+-release channel of the sarcoplasmic reticulum (SR). In both fast- and slow-type fibers, identified by their tension response to pSr 5.0, BSA (0.7-15 µM) had no effect on the Ca2+ affinity of the contractile proteins and elicited no tension per se in Ca2+-loaded fibers. In contrast, BSA (&amp;gt;1.0 µM) potentiated the caffeine-induced tension in Ca2+-loaded fibers, this effect being more intense in slow-type fibers. Thus, BSA reduced the threshold caffeine concentration required for eliciting detectable tension, and increased the amplitude, the rate of rise and the area under the curve of caffeine-induced tension. BSA also potentiated the tension elicited in Ca2+-loaded fibers by low-Mgv solutions containing 1.0 mM free ATP. These results suggest that BSA modulates the response of the human skeletal muscle SR Ca2+-release channel to activators such as caffeine and ATP.
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500017
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1997000500017
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.30 n.5 1997
Fonte: | info:eu-repo/semantics/openAccess