Data/hora: 09/06/2024 07:48:47

Provedor de dados: | 56
País: | Brazil
Título: | Glucagon-like peptide 1 improves insulin resistance in vitro through anti-inflammation of macrophages
Autores: | Guo,C.; Huang,T.; Chen,A.; Chen,X.; Wang,L.; Shen,F.; Gu,X.
Data: | 2016-01-01
Ano: | 2016
Palavras-chave: | Diabetes; Glucagon-like peptide; Macrophage infiltration; Adipose inflammation; Insulin resistance.
Resumo: | Glucagon-like peptide 1 (GLP-1), a kind of gut hormone, is used in the treatment of type 2 diabetes (T2D). Emerging evidence indicates that GLP-1 has anti-inflammatory activity. Chronic inflammation in the adipose tissue of obese individuals is a cause of insulin resistance and T2D. We hypothesized that GLP-1 analogue therapy in patients with T2D could suppress the inflammatory response of macrophages, and therefore inhibit insulin resistance. Our results showed that GLP-1 agonist (exendin-4) not only attenuated macrophage infiltration, but also inhibited the macrophage secretion of inflammatory cytokines including TNF-β, IL-6, and IL-1β. Furthermore, we observed that lipopolysaccharide (LPS)-induced macrophage conditioned media could impair insulin-stimulated glucose uptake. This effect was compensated by treatment with the conditioned media from macrophages treated with the combination of LPS and exendin-4. It was also observed that exendin-4 directly inhibited the activation of NF-κB in macrophages. In conclusion, our results indicated that GLP-1 improved inflammatory macrophage-derived insulin resistance by inhibiting NF-κB pathway and secretion of inflammatory cytokines in macrophages. Furthermore, our observations suggested that the anti-inflammatory effect of GLP-1 on macrophages can contribute to GLP-1 analogue therapy of T2D.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001200602
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431x20165826
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.49 n.12 2016
Fonte: | info:eu-repo/semantics/openAccess