Data/hora: 05/06/2024 04:22:35

Provedor de dados: 	56	
País: 	Brazil	
Título: 	In vitro activity of hypnophilin from Lentinus strigosus: a potential prototype for Chagas disease and leishmaniasis chemotherapy	
Autores: 	Souza-Fagundes,E.M.; Cota,B.B.; Rosa,L.H.; Romanha,A.J.; Corrêa-Oliveira,R.; Rosa,C.A.; Zani,C.L.; Teixeira-Carvalho,A.; Martins-Filho,O.A.	
Data: 	2010-11-01	
Ano: 	2010	
Palavras-chave: 	Chagas disease; Leishmaniasis; Chemotherapy; Immunomodulation; Hypnophilin; Lentinus strigosus.	
Resumo: 	Hypnophilin and panepoxydone, terpenoids isolated from Lentinus strigosus, have significant inhibitory activity onTrypanosoma cruzi trypanothione reductase (TR). Although they have similar TR inhibitory activity at 10 μg/mL (40.3 μM and 47.6 μM for hypnophilin and panepoxydone, respectively; ~100%), hypnophilin has a slightly greater inhibitory activity (~71%) on T. cruzi amastigote (AMA) growth in vitro as well as on in vitro phytohemagglutinin (PHA)-induced peripheral blood mononuclear (PBMC) proliferation (~70%) compared to panepoxydone (69% AMA inhibition and 91% PBMC inhibition). Hypnophilin and panepoxydone at 1.25 μg/mL had 67% inhibitory activity onLeishmania (Leishmania) amazonensis amastigote-like (AMA-like) growth in vitro. The panepoxydone activity was accompanied by a significant inhibitory effect on PHA-induced PBMC proliferation, suggesting a cytotoxic action. Moreover, incubation of human PBMC with panepoxydone reduced the percentage of CD16+ and CD14+ cells and down-regulated CD19+, CD4+ and CD8+ cells, while hypnophilin did not alter any of the phenotypes analyzed. These data indicate that hypnophilin may be considered to be a prototype for the design of drugs for the chemotherapy of diseases caused by Trypanosomatidae.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100007	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/S0100-879X2010007500108	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.43 n.11 2010	
Fonte: 	info:eu-repo/semantics/openAccess