Data/hora: 09/06/2024 10:38:24

Provedor de dados: | 56
País: | Brazil
Título: | A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins
Autores: | Rocha,H.A.O.; Franco,C.R.C.; Trindade,E.S.; Carvalho,L.C.M.; Veiga,S.S.; Leite,E.L.; Dietrich,C.P.; Nader,H.B.
Data: | 2001-05-01
Ano: | 2001
Palavras-chave: | CHO cells; Extracellular matrix proteins; Fucan; Glycosaminoglycans; Cell adhesion; Brown algae.
Resumo: | Fucans, a family of sulfated polysaccharides present in brown seaweed, have several biological activities. Their use as drugs would offer the advantage of no potential risk of contamination with viruses or particles such as prions. A fucan prepared from Spatoglossum schröederi was tested as a possible inhibitor of cell-matrix interactions using wild-type Chinese hamster ovary cells (CHO-K1) and the mutant type deficient in xylosyltransferase (CHO-745). The effect of this polymer on adhesion properties with specific extracellular matrix components was studied using several matrix proteins as substrates for cell attachment. Treatment with the polymer inhibited the adhesion of fibronectin to both CHO-K1 (2 x 10(5))()and CHO-745 (2 x 10(5) and 5 x 10(5)) cells. No effect was detected with laminin, using the two cell types. On the other hand, adhesion to vitronectin was inhibited in CHO-K1 cells and adhesion to type I collagen was inhibited in CHO-745 cells. In spite of this inhibition, the fucan did not affect either cell proliferation or cell cycle. These results demonstrate that this polymer is a new anti-adhesive compound with potential pharmacological applications.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500009
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2001000500009
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.34 n.5 2001
Fonte: | info:eu-repo/semantics/openAccess