Data/hora: 14/06/2024 02:19:14

Provedor de dados: | 56
País: | Brazil
Título: | Immunization against the colonization factor antigen I of enterotoxigenic Escherichia coli by administration of a bivalent Salmonella typhimurium aroA strain
Autores: | Guillobel,H.C.R.; Luna,M.G.; Camacho,E.F.; Almeida,D.F.; Ferreira,L.C.S.
Data: | 1998-04-01
Ano: | 1998
Palavras-chave: | Salmonella typhimurium aroA; Enterotoxigenic Escherichia coli; Attenuated vaccines; Colonization factor antigen I.
Resumo: | An expression plasmid (pCFA-1) carrying the cfaB gene that codes for the enterotoxigenic Escherichia coli (ETEC) fimbrial adhesin colonization factor antigen I (CFA/I) subunit was constructed and used to transform a derivative of the attenuated Salmonella typhimurium aroA vaccine strain SL3261 carrying an F'lacIq. Treatment of the transformed strain with isopropyl-ß-D-thiogalactopyranoside (IPTG) resulted in elevated in vitro expression of the CFA/I subunit. Although flagellar function and lipopolysaccharide (LPS) synthesis were similar in both the parental and the recombinant strains, spleen colonization was reduced in the recombinant strain. All BALB/c mice parenterally inoculated with the recombinant strain developed significant anti-CFA/I and anti-LPS serum antibody titers (P&amp;lt;0.05). Moreover, 2 of 5 mice orally inoculated with the engineered Salmonella strain developed anti-CFA/I intestinal IgA (P&amp;gt;0.05) while 4/5 of the same mice developed anti-LPS IgA (P&amp;lt;0.05). The results indicate that the vaccine strain elicited an antibody response against the bacterial host both after oral and intravenous immunization while the response against the CFA/I antigen was significant only after inoculation by the intravenous route
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400012
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1998000400012
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.31 n.4 1998
Fonte: | info:eu-repo/semantics/openAccess