Data/hora: 01/06/2024 11:50:23

Provedor de dados: | 56
País: | Brazil
Título: | Neonatal handling induces anovulatory estrous cycles in rats
Autores: | Gomes,C.M.; Frantz,P.J.; Sanvitto,G.L.; Anselmo-Franci,J.A.; Lucion,A.B.
Data: | 1999-10-01
Ano: | 1999
Palavras-chave: | Stress; Neonatal handling; Estrous cycle; Ovulation; Female rat.
Resumo: | Since previous work has shown that stimulation early in life decreases sexual receptiveness as measured by the female lordosis quotient, we suggested that neonatal handling could affect the function of the hypothalamus-pituitary-gonadal axis. The effects of neonatal handling on the estrous cycle and ovulation were analyzed in adult rats. Two groups of animals were studied: intact (no manipulation, N = 10) and handled (N = 11). Pups were either handled daily for 1 min during the first 10 days of life or left undisturbed. At the age of 90 days, a vaginal smear was collected daily at 9:00 a.m. and analyzed for 29 days; at 9:00 a.m. on the day of estrus, animals were anesthetized with thiopental (40 mg/kg, ip), the ovaries were removed and the oviduct was dissected and squashed between 2 glass slides. The number of oocytes of both oviductal ampullae was counted under the microscope. The average numbers for each phase of the cycle (diestrus I, diestrus II, proestrus and estrus) during the period analyzed were compared between the two groups. There were no significant differences between intact and handled females during any of the phases. However, the number of handled females that showed anovulatory cycles (8 out of 11) was significantly higher than in the intact group (none out of 10). Neonatal stimulation may affect not only the hypothalamus-pituitary-adrenal axis, as previously demonstrated, but also the hypothalamus-pituitary-gonadal axis in female rats.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000010
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1999001000010
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.32 n.10 1999
Fonte: | info:eu-repo/semantics/openAccess