Data/hora: 11/06/2024 10:57:53

Provedor de dados: | 56
País: | Brazil
Título: | Large-scale production and purification of recombinant protein from an insect cell/baculovirus system in Erlenmeyer flasks: application to the chicken poly(ADP-ribose) polymerase catalytic domain
Autores: | Miranda,E.A.; de-Murcia,G.; Ménissier-de-Murcia,J.
Data: | 1997-08-01
Ano: | 1997
Palavras-chave: | PARP; Insect cells; Baculovirus; Overproduction.
Resumo: | A simple and inexpensive shaker/Erlenmeyer flask system for large-scale cultivation of insect cells is described and compared to a commercial spinner system. On the basis of maximum cell density, average population doubling time and overproduction of recombinant protein, a better result was obtained with a simpler and less expensive bioreactor consisting of Erlenmeyer flasks and an ordinary shaker waterbath. Routinely, about 90 mg of pure poly(ADP-ribose) polymerase catalytic domain was obtained for a total of 3 x 109 infected cells in three liters of culture
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800002
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1997000800002
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.30 n.8 1997
Fonte: | info:eu-repo/semantics/openAccess