Data/hora: 11/06/2024 12:36:13

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Modulation of the expression of the transcription factor Max in rat retinal ganglion cells by a recombinant adeno-associated viral vector	
Autores: 	Petrs-Silva,H.; Chiodo,V.; Chiarini,L.B.; Hauswirth,W.W.; Linden,R.	
Data: 	2005-03-01	
Ano: 	2005	
Palavras-chave: 	Retina; Max expression; Transcription factor; Viral vector; Gene therapy; Neuroprotection.	
Resumo: 	Exclusion of the transcription factor Max from the nucleus of retinal ganglion cells is an early, caspase-independent event of programmed cell death following damage to the optic axons. To test whether the loss of nuclear Max leads to a reduction in neuroprotection, we developed a procedure to overexpress Max protein in rat retinal tissue in vivo. A recombinant adeno-associated viral vector (rAAV) containing the max gene was constructed, and its efficiency was confirmed by transduction of HEK-293 cells. Retinal ganglion cells were accessed in vivo through intravitreal injections of the vector in rats. Overexpression of Max in ganglion cells was detected by immunohistochemistry at 2 weeks following rAAV injection. In retinal explants, the preparation of which causes damage to the optic axons, Max immunoreactivity was increased after 30 h in vitro, and correlated with the preservation of a healthy morphology in ganglion cells. The data show that the rAAV vector efficiently expresses Max in mammalian retinal ganglion cells, and support the hypothesis that the Max protein plays a protective role for retinal neurons.	
Tipo: 	Info:eu-repo/semantics/other	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300008	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/S0100-879X2005000300008	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.38 n.3 2005	
Fonte: 	info:eu-repo/semantics/openAccess