Data/hora: 07/06/2024 08:49:58

Provedor de dados: | 56
País: | Brazil
Título: | Rat visceral yolk sac cells: viability and expression of cell markers during maternal diabetes
Autores: | Aires,M.B.; Santos,J.R.A.; Souza,K.S.; Farias,P.S.; Santos,A.C.V.; Fioretto,E.T.; Maria,D.A.
Data: | 2015-08-01
Ano: | 2015
Palavras-chave: | Visceral yolk sac; Diabetes; Embryo; Pregnancy; Cell markers.
Resumo: | The function of the visceral yolk sac (VYS) is critical for embryo organogenesis until final fetal development in rats, and can be affected by conditions such as diabetes. In view of the importance of diabetes during pregnancy for maternal and neonatal health, the objective of this study was to assess fetal weight, VYS cell markers, and viability in female Wistar rats (200-250 g) with induced diabetes (alloxan, 37 mg/kg) on the 8th gestational day (gd 8). At gd 15, rats from control (n=5) and diabetic (n=5) groups were anesthetized and laparotomized to remove the uterine horns for weighing of fetuses and collecting the VYS. Flow cytometry was used for characterizing VYS cells, and for determining mitochondrial activity, cell proliferation, DNA ploidy, cell cycle phases, and caspase-3 activity. Fetal weight was reduced in the diabetic group. Expression of the cell markers CD34, VEGFR1, CD115, CD117, CD14, CCR2, CD90, CD44, STRO-1, OCT3/4, and Nanog was detected in VYS cells in both groups. In the diabetic group, significantly decreased expression of CD34 (P&lt;0.05), CCR2 (P&lt;0.001), and OCT3/4 (P&lt;0.01), and significantly increased expression of CD90 (P&lt;0.05), CD117 (P&lt;0.01), and CD14 (P&lt;0.05) were observed. VYS cells with inactive mitochondria, activated caspase-3, and low proliferation were present in the rats with diabetes. Severe hyperglycemia caused by maternal diabetes had negative effects on pregnancy, VYS cell viability, and the expression of cell markers.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800676
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431x20154739
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.48 n.8 2015
Fonte: | info:eu-repo/semantics/openAccess