Data/hora: 06/06/2024 06:34:53

Provedor de dados: | 56
País: | Brazil
Título: | Thyroid hormone regulates protein expression in C6 glioma cells
Autores: | Trentin,A.G.; Alvarez-Silva,M.
Data: | 1998-10-01
Ano: | 1998
Palavras-chave: | Thyroid hormone; Glioma; Protein synthesis; GFAP; Vimentin; Differentiation.
Resumo: | Thyroid hormone (T3) is essential to normal brain development. Previously, we have shown that T3 induces cerebellar astrocyte proliferation. This effect is accompanied by alteration in glial fibrillary acidic protein (GFAP) and fibronectin organization. In the present study, we report that the C6 glioma cell line, which expresses GFAP and is classified as an undifferentiated astrocytic cell type, is a target for T3 action. The C6 monolayers were treated with 50 nM T3 for 3 days, after which the cells were maintained for 2 days without medium changes. In C6 cells, T3 induced the expression of proteins of 107, 73 and 62 kDa. The hormone also up-regulated protein bands of 100 (+50%), 37 (+50%) and 25.5 kDa (+50%) and down-regulated proteins of 94 (-100%), 86.5 (-100%), 68 (-100%), 60 (-100%), 54 (-33%), 51 (-33%) and 43.5 kDa (-33%). We suggest, on the basis of molecular mass, that the 54-, 51- and 43.5-kDa proteins could be the cytoskeletal proteins vimentin, GFAP and actin, respectively. The down-regulation of these proteins may be involved in the effects of thyroid hormone on C6 differentiation.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000008
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1998001000008
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.31 n.10 1998
Fonte: | info:eu-repo/semantics/openAccess