Data/hora: 01/06/2024 01:33:52

Provedor de dados: | 56
País: | Brazil
Título: | Aggrecan structure in amphibian cartilage
Autores: | Covizi,D.Z.; Carvalho,H.F.
Data: | 2000-12-01
Ano: | 2000
Palavras-chave: | Aggrecan; Bullfrog; Cartilage; Chondroitin sulfate; Epiphyseal cartilage.
Resumo: | The structure of the large proteoglycan present in the bullfrog epiphyseal cartilage was studied by immunochemical and biochemical methods. The isolated monomer showed a polydisperse behavior on Sepharose CL2B, with a peak at Kav = 0.14. Chondroitin sulfate chains were identified by HPLC analysis of the products formed by chondroitinase digestion and mercuric acetate treatment. These chains have approximately 38 disaccharides, a Di45:Di68 ratio of 1.6 and GalNAc4S + GalNAc4,6S are the main non-reducing terminals. Keratan sulfate was identified by the use of two monoclonal antibodies in Western blots after chondroitinase ABC treatment. A keratan sulfate-rich region (~110 kDa) was isolated by sequential treatment with chondroitinase ABC and proteases. We also employed antibodies in Western blotting experiments and showed that the full length deglycosylated core protein is about 300 kDa after SDS-PAGE. Domain-specific antibodies revealed the presence of immunoreactive sites corresponding to G1/G2 and G3 globular domains and the characterization of this large proteoglycan as aggrecan. The results indicate the high conservation of the aggrecan domain structure in this lower vertebrate.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200002
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2000001200002
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.33 n.12 2000
Fonte: | info:eu-repo/semantics/openAccess