Data/hora: 14/06/2024 02:36:11

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Leptin receptor (Ob-R) mRNA expression and serum leptin concentration in patients with colorectal and metastatic colorectal cancer	
Autores: 	Erkasap,N.; Ozkurt,M.; Erkasap,S.; Yasar,F.; Uzuner,K.; Ihtiyar,E.; Uslu,S.; Kara,M.; Bolluk,O.	
Data: 	2013-03-01	
Ano: 	2013	
Palavras-chave: 	Leptin; Colorectal cancer; Metastatic colorectal cancer; Leptin receptor (Ob-R); Real-time PCR.	
Resumo: 	The objective of the present study was to investigate the effect of leptin on the progression of colorectal carcinoma to metastatic disease by analyzing the serum leptin concentration and Ob-R gene expression in colon cancer tissues. Tissue samples were obtained from 31 patients who underwent surgical resection for colon (18 cases) and metastatic colon (13 cases) cancer. Serum leptin concentration was determined by an enzyme-linked immunosorbent assay (ELISA) and Ob-R mRNA expression by real-time polymerase chain reaction (RT-PCR) for both groups. ELISA data were analyzed by the Student t-test and RT-PCR data were analyzed by the Mann-Whitney U-test. RT-PCR results demonstrated that mRNA expression of Ob-R in human metastatic colorectal cancer was higher than in local colorectal cancer tissues. On the other hand, mean serum leptin concentration was significantly higher in local colorectal cancer patients compared to patients with metastatic colorectal cancer. The results of the present study suggest a role for leptin in the progression of colon cancer to metastatic disease without weight loss. In other words, significantly increased Ob-R mRNA expression and decreased serum leptin concentration in patients with metastatic colon cancer indicate that sensitization to leptin activity may be a major indicator of metastasis to the colon tissue and the determination of leptin concentration and leptin gene expression may be used to aid the diagnosis.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300306	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/1414-431X20122559	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.46 n.3 2013	
Fonte: 	info:eu-repo/semantics/openAccess