Data/hora: 06/06/2024 13:32:39

Provedor de dados: | 56
País: | Brazil
Título: | IgE expression on the surface of B1 and B2 lymphocytes in experimental murine schistosomiasis
Autores: | Oliveira,F.L.; Aguiar,A.M.; Borojevic,R.; El-Cheikh,M.C.
Data: | 2005-07-01
Ano: | 2005
Palavras-chave: | Schistosomiasis; B lymphocytes; Immunoglobulin switch.
Resumo: | In a previous study we monitored the distribution and phenotype expression of B1 cells during the evolution of experimental murine schistosomiasis mansoni and we proposed that the B1 cells were heterogeneous: a fraction which originated in the spleen and followed the migratory pathway to mesenteric ganglia, while the other was the resident peritoneal B1-cell pool. In the present study, we have addressed the question of whether these two B1-lymphocyte populations are involved in the production of the late Ig isotype IgE, which is present in high levels in schistosomal infection. Lymphocyte expression of surface markers and immunoglobulins were monitored by immunofluorescence flow cytometry. Both in the spleen and mesenteric ganglia, the B1 and B2 cells were induced to switch from IgM to IgE in the early Th2-dominated phase of the disease, with an increase of IgE in its later phases. Conversely, peritoneal B1-IgM+ switched to the remaining IgE+ present in high numbers in the peritoneal cavity throughout the disease. We correlated the efficient induction of the expression of late Ig isotypes by B1 cells with high levels of inflammatory cytokines due to the intense host response to the presence of worms and their eggs in the abdominal cavity. In conclusion, B1 cells have a different switch behavior from IgM to IgE indicating that these cell sub-populations depend on the microenvironment.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700006
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2005000700006
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.38 n.7 2005
Fonte: | info:eu-repo/semantics/openAccess