Data/hora: 05/06/2024 08:14:40

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Stimulated mast cells promote maturation of myocardial microvascular endothelial cell neovessels by modulating the angiopoietin-Tie-2 signaling pathway	
Autores: 	Wang,Z.H.; Zhu,W.; Tao,J.P.; Zhang,Q.Y.; Wei,M.	
Data: 	2013-10-01	
Ano: 	2013	
Palavras-chave: 	Myocardial microvascular endothelial cells; Mast cell; Angiogenesis; Angiopoietin; Tie-2.	
Resumo: 	Angiopoietin (Ang)-1 and Ang-2 interact in angiogenesis to activate the Tie-2 receptor, which may be involved in new vessel maturation and regression. Mast cells (MCs) are also involved in formation of new blood vessels and angiogenesis. The present study was designed to test whether MCs can mediate angiogenesis in myocardial microvascular endothelial cells (MMVECs). Using a rat MMVEC and MC co-culture system, we observed that Ang-1 protein levels were very low even though its mRNA levels were increased by MCs. Interestingly, MCs were able to enhance migration, proliferation, and capillary-like tube formation, which were associated with suppressed Ang-2 protein expression, but not Tie-2 expression levels. These MCs induced effects that could be reversed by either tryptase inhibitor [N-tosyl-L-lysine chloromethyl ketone (TLCK)] or chymase inhibitor (N-tosyl-L-phenylalanyl chloromethyl ketone), with TLCK showing greater effects. In conclusion, our data indicated that MCs can interrupt neovessel maturation via suppression of the Ang-2/Tie-2 signaling pathway.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100920	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/1414-431X20132873	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.46 n.11 2013	
Fonte: 	info:eu-repo/semantics/openAccess